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Image Search Results
Journal: Journal of Translational Medicine
Article Title: Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas
doi: 10.1186/1479-5876-10-209
Figure Lengend Snippet: DLEC1 is downregulated in lymphoma cell lines. A. Expression analysis of DLEC1 in normal adult tissues, lymph nodes, and PBMC samples. B. Representative analyses of DLEC1 expression by RT-PCR in lymphoma cell lines. GAPDH was used as internal control. B.M: bone marrow; DLBCL: diffuse large B-cell lymphoma; PTCL: peripheral T cell lymphoma; BL: Burkitt lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma.
Article Snippet: Non-Hodgkin and Hodgkin lymphoma cell lines studied included
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control
Journal: Journal of Translational Medicine
Article Title: Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas
doi: 10.1186/1479-5876-10-209
Figure Lengend Snippet: Promoter CpG methylation of DLEC1 in lymphoma cell lines. A. Diagram shows the DLEC1 CpG island, exon 1 (indicated with a rectangle), and CpG sites (short vertical lines). Numbers from 1 to 61 indicate the individual CpG sites. B. DLEC1 methylation as measured by MSP in lymphoma cell lines. M: methylated; U: unmethylated; DLBCL: diffuse large B-cell lymphoma; BL: Burkitt lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma. C. High resolution mapping of the methylation status of the DLEC1 promoter by BGS in normal PBMCs, and representative BL and HL cell lines. The DLEC1 transcription start site is shown as bent arrows. Circles, CpG sites analyzed; row of circles, an individual promoter allele that was cloned, randomly selected, and sequenced; filled circle, methylated CpG site; open circle, unmethylated CpG site.
Article Snippet: Non-Hodgkin and Hodgkin lymphoma cell lines studied included
Techniques: CpG Methylation Assay, Methylation, Clone Assay
Journal: Journal of Translational Medicine
Article Title: Epigenetic silencing of the 3p22 tumor suppressor DLEC1 by promoter CpG methylation in non-Hodgkin and Hodgkin lymphomas
doi: 10.1186/1479-5876-10-209
Figure Lengend Snippet: DLEC1 is frequently methylated in lymphoma tissues. A. MSP analysis of DLEC1 methylation in normal lymph node samples. Templates used for positive controls are: bisulfite treated DNA from HCT116 (+ve-control1) and HT-29 (+ve-control2) cell lines for methylation; SW480 (+ve-control1) and SNU16 (+ve-control2) cell lines for unmethylation. B. Representative analyses of DLEC1 methylation in primary lymphomas by MSP in primary lymphoma BL, DLBCL, HL and FL samples. BL: Burkitt lymphoma; DLBCL: diffuse large B-cell lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma; FL: follicular lymphoma. C. Representative analysis of DLEC1 expression in NL tumors. GAPDH was used as an internal control. D. Representative MSP analysis of DLEC1 methylation in serum samples from HL patients and normal controls. Positive controls: bisulfite treated DNA from HCT116 (+ve-control1) and HT-29 (+ve-control2) cell lines. M: methylated; NS: non-specific band.
Article Snippet: Non-Hodgkin and Hodgkin lymphoma cell lines studied included
Techniques: Methylation, Expressing, Control